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Characteristics of the included studies. For each study, the aim, study design, participants, type of intervention, outcome measures and main results are reported.

Journal: Nutrients

Article Title: Relationship Between the Gut Microbiome, Tryptophan-Derived Metabolites, and Osteoarthritis-Related Pain: A Systematic Review with Meta-Analysis

doi: 10.3390/nu17020264

Figure Lengend Snippet: Characteristics of the included studies. For each study, the aim, study design, participants, type of intervention, outcome measures and main results are reported.

Article Snippet: Boer et al. [ ] , The Rotterdam Study aimed to examine the connection between gastrointestinal microbiome composition and joint pain, specifically focusing on knee pain associated with osteoarthritis (OA) , Observational study (case–control) , 1427 patients (821 women), mean age 56.8 ( ± 5.9) years.Inclusion criteria: patients with knee OA(cases) and without knee OA (controls) fromthe Rotterdam Study , 16S rRNA gene-based Illumina sequencingfor microbiome profiling; WOMAC index , Results indicated a significant link between microbiome beta diversity and knee WOMAC scores. Subjects reporting higher WOMAC pain had increased Streptococcus abundance, regardless of tobacco use, alcohol consumption, and BMI. The relative abundance of Streptococcus spp. strongly correlated with WOMAC knee pain scores ( p = 1.4 × 10 −4 ), attributed to localized knee joint inflammation..

Techniques: DNA Extraction, Sequencing, Amplification, Multiplex Assay, Control, Sandwich ELISA